Difference Between Dulbecco’s PBS and PBS

DPBS vs PBS

PBS, or phosphate buffered saline, and Dulbecco’s phosphate buffered saline (DPBS), are buffered salines used in biological studies. PBS and DPBS are used in research involving cells. The solution’s ion concentration and osmolarity is isotonic, that is, it is compatible with the human body. The buffer facilitates to provide and preserve a stable pH of 7.2-7.6.

There is no significant difference between PBS and DPBS. Both of them contain sodium phosphate, sodium chloride, and, when required, potassium phosphate and potassium chloride. Other preparations, such as DPBS, may or may not contain calcium and magnesium. PBS and DPBS have numerous applications because they are not noxious to cells. Both PBS and DPBS can be used to rinse instruments or containers contaminated with cells. Also, both of them can be used in diluting substances. Water molecules within diluents like protein are halted and dried up. The water that combines with the substance keeps the substance from undergoing conformational alterations such as denaturation. Calcium and magnesium solutions may restrain the activity of trypsin. In ellipsometry, the adsorption of protein can be measured by taking a basis spectrum with the use of PBS.

Phosphate buffered saline with additives such as EDTA can be used to separate bundled cells. Precipitation is formed when zinc, a divalent metal, is added to PBS which is why it cannot be added to uncouple cells. Good’s buffer is used instead of zinc in this type of situation.

Phosphate buffered saline and Dulbecco’s phosphate buffered saline are prepared in numerous ways. One way to prepare it is by dissolving 800 g. sodium chloride, 20 g. potassium chloride, 2 molecules of water, 24 g. potassium phosphate, and 144 g. sodium phosphate in 8 L. of distilled water. The outcome will be 10 liters of 10x PBS. Directly measure the pH of the PBS using a pH meter when preparing buffered solutions. The pH of the solution is adjustable by using sodium hydroxide or hydrochloric acid. Phosphate buffered saline as a diluent is prepared with a concluding concentration of 10 mM Phosphate, 137 mM Sodium chloride, 2.7 mM potassium chloride, and a pH of 7.4. Fritsch, Sambrook, and Maniatis have their own way in preparing buffer solutions in their book titled “Molecular Cloning.” Accordingly, for a liter of 1x PBS, begin the preparation with 800 ml. of water that is distilled. Include 8 g. of sodium chloride as well as 0.2 g. of potassium chloride. 1.44 g. of sodium phosphate is then added followed by 0.24 g. of potassium phosphate. Put in hydrochloric acid to adjust the pH to 7.4. To come up with 1 liter of PBS, add more distilled water until leveling up to 1 liter. Dole out the resulting solution into an aliquot and use the autoclave to sterilize the solution. Adjust the autoclave to 121˚ C and autoclave it for 20 minutes in its liquid cycle. Afterwards, store the solution at room temperature.

Although phosphate buffered saline and Dulbecco’s phosphate buffered saline are prepared in many ways, the contents are still the same. Therefore, no matter how it is being prepared, the results and the uses are still the same.

Summary:

1.PBS, or phosphate buffered saline, and Dulbecco’s phosphate buffered saline (DPBS), are buffered salines used in biological studies.

2.The solution’s ion concentration and osmolarity is isotonic, that is, it is compatible with the human body. The buffer facilitates to provide and preserve a stable pH of 7.2-7.6.

3.There is no significant difference between PBS and DPBS. Both of them contain sodium phosphate, sodium chloride, and, when required, potassium phosphate and potassium chloride. Other preparations, such as DPBS, may or may not contain calcium and magnesium.

4.Both PBS and DPBS can be used to rinse instruments or containers contaminated with cells. Also, both of them can be used in diluting substances. Water molecules within diluents like protein are halted and dried up. The water that combines with the substance keeps the substance from undergoing conformational alterations such as denaturation. Calcium and magnesium solutions may restrain the activity of trypsin. In ellipsometry, the adsorption of protein can be measured by taking a basis spectrum with the use of PBS.

5.Phosphate buffered saline with additives such as EDTA can be used to separate bundled cells. Precipitation is formed when zinc, a divalent metal, is added to PBS which is why it cannot be added to uncouple cells. Good’s buffer is used instead of zinc in this type of situation.

6.PBS and DPBS are prepared in numerous ways. Although phosphate buffered saline and Dulbecco’s phosphate buffered saline are prepared in many ways, the contents are still the same. Therefore, no matter how it is being prepared, the results and the uses are still the same.

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